Fas counterattack in cholangiocarcinoma: a mechanism for immune evasion in human hilar cholangiocarcinomas

摘要

Conclusion: Hilar cholangiocarcinomas may elude immunological surveillance by inducing, via Fas/FasL system, the apoptosis of activated lymphocytes. Aim: To investigate FasL expression in hilar Cholangiocarcinoma tissues and cultured Cholangiocarcinoma cells, and to assess its ability to induce Apoptosis. Methods: We studied the expression of FasL by humnan Hilar cholangiocaroinomas tissues by Immunohistochemistry, ant the QBC939 cholangiocarcinoma Cell line by RT-PCR, immunohistochemistry, and Western Blot. TUNEL and flow cytometry were used to detect Apoptotic cells.