Telomere and telomerase in the initial stage of immortalization of seophageal epithelial cell

摘要

Conclusion: At the early stage of SHEE cells, telomeres are shortened without expression of telomerase and hTERT causing cellular senescence and cell death. From the 20 th to the 30 th passages, the activation of telomerase and maintenance of telomere length show a progressive process for immotalization of esophageal epithelial cells. the expression of telomerase may constitute a biomarker for detection of immortalization of cells. Aim: To search for the biomarker of cellular immortalization, the telomere length, telomerase activity and its subunits in cultured epithelial cells of human fetal esophagus in the process of immortalization.