Mesenteric artery remodeling and effects of imidapril and irbesartan on it in spontaneously hypertensive rats

摘要

AIM: To investigate the remodeling of mesenteric artery and the expression of TGF-β_1, c-Jun in mesenteric artery and effects of imidapril and irbesartan on the remodeling in spontaneously hypertensive rats (SHR). METHODS: Thirty SHR (male/female, 21/9), aged 13 wk, were randomly divided into 3 groups (7 male rats and 3 female rats each group): SHR group, imidapril group (imidapril 3 mg/kg·d was given in drinking water for 14 wk), and irbesartan group (irbesartan 50 mg/kg·d was given in drinking water foe 14 wk). Ten homogenous Wistar Kyoto rats, 5 males and 5 females, weighing 206 +- 49 g, were selected as normal control group (WKY group). Systolic pressure was measured on d 1, 2, 4, 6, 8, 10, 12 and 14 during the experiment and the rats were killed at the end of the experiment. Angiotensin Ⅱ (Ang Ⅱ) level in plasma and mesenteric arteries was measured by radioimmunoassay. The morphology of the secondary branches of mesenteric artery were examined by light microscopy and electron microscopy. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of transforming growth factor TGF-β_1 and c-Jun mRNA. RESULTS: Compared with imidapril group and irbesartan group, the blood pressure was remarkably increased in SHR group. Ang II level in plasma and mesenteric arteries in SHR group was the same or lower than that in WKY group, and was higher in irbesartan group and lower in imidapril group. The remodeling of mesenteric arteries in SHR group was mostly obvious among the 4 groups. The ratio of TGF-β_1 absorbed light value to GAPDH absorbed light value in the SHR group was 0.887 +- 0.019, which was significantly higher than that in WKY group, imidapril group, and irbesartan group with the ratios of 0.780 +- 0.018, 0.803 +- 0.005, and 0.847 +- 0.017, respectively (P<0.01). Ang Ⅱ level in plasma and mesenteric arteries in imidapril group was significantly lower than that in irbesartan group (P<0.05). The c-Jun absorbed light value/GAPDH absorbed light value of mesenteric arteries in the SHR group was 0.850 +- 0.015, which was significantly higher than that in the WKY, imidapril, and irbesartan groups (0.582 +- 0.013, 0.743 +- 0.012, and 0.789 +- 0.013, respectively, P<0.01), and was significantly lower in imidapril group than in irbesartan group (P<0.05). CONCLUSION: Imidapril and irbesartan can not only control blood pressure but also inhibit mesenteric arteries remodeling and mRNA expression of TGF-β_1, c-Jun in SHR. Imidapril is more effective than irbesartan.