By 19641 was thoroughly committed to Gaddum's technique of superfusion (Gaddum 1953) and also to the principles of parallel pharmacological assay proposed by Gaddum (1959) as strong evidence for the identity of a compound. I introduced the idea of superfusing several tissues in cascade (generally up to six, arranged in two banks). Besides enabling the parallel assay of individually injected samples, this arrangement also allowed parallel and dynamic analysis of the active components present in a fluid stream taken from the outflow of a perfused organ. Many different buffers such as Kreb's solution, Tyrode's solution, Locke's solution etc., are used to support the activity of isolated organs. Vane's solution was something entirely different! All of these artificial solutions were based on the concentrations of various salts in the blood, so why not use blood itself? This idea was the birth of what became known as the blood-bathed organ technique. An anaesthetised animal is heparinized and arterial blood is removed at a constant rate of 10-15 ml per minute (dogs, cats or rabbits). Lower rates were used from guinea-pigs (Piper, Collier and Vane 1967). The blood superfuses the cascade of tissues and is then returned intravenously to the animal.
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