Effects of the neuroprotectant lubeluzole on the cytotoxic actions of veratridine, barium, ouabain and 6-hydroxydopamine in chromaffin cells

摘要

Incubation of bovine adrenal chromaffin cells with veratridine(10-100 μM) during 24 h, caused a concentration-dependent release of the cytosolic lactate dehydrogenase(LHD) into the bathing medium, an indicator of cell death. Lubeluzole or its R(-) enantiomer, R91154, did not enhance LDH release. Both lubeluzole and R91154(0.3-10 μM) decreased the veratridine-induced LDH release. Penfluridol did not increase LDH release at concentrations 0.003-1μM; 3-10μM increased LDH Release to 50-60/100, after 24 h exposure. Penfluridol (0.03-0.3 μM) did not protect against the cytotoxic Effects of veratridine; at 1 μM, 15/100 protection was produced. Higher concentrations (3-10μM) Enhanced the cytotoxic effects of veratridine.