Effect of synthetic polyelectrolytes on enhanced chemiluminescence reaction in solution

摘要

The coupled peroxidase-catalysed chemiluminescence reaction of luminol and p-iodophenol oxidation of H_2O_2 (the 'enhanced-chemiluminescence reaction') was studied in the presence of synthetic polyelectrolytes with various chemical structures. An addition of both aliphatic poly-(NN'-dimethyldiallylainmonium chloride) (PDM) polycation and aromatic poly-(N-ethyl-4-vinylpyridinium bromide) polycation (PEVP) in the reaction mixture led to an increase in the kinetic constants for the elementary enzymic reaction of luminol oxidation. By contrast, whereas the addition of PDM gave rise to an increase in chemiluminescence light intensity (I_(CL)). the addition of PEVP caused a decrease in the signal. The results of a study of a model non-enzymic reaction proved that the observed decrease of I_(CL) is due to the interaction of PEVP pyridinium groups with the intermediate products of luminol oxidation. The most pronounced decrease of I_(CL) was in the case of the polyelectrolyte complex formed by PEVP polycation and the conjugate of peroxidase with poly(methacrylic acid) (polyanion). The demonstrated ability of synthetic polyelectrolytes to effect both enzymic and non-enzymic stages of the reaction could be used in the development of new immunoassays of biologically active substances.