Ceriporia lacerata DMC1106, a New Endophytic Fungus: Isolation, Identification, and Optimal Medium for 2',4'-dihydroxy-6'-methoxy-3',5 '-dimethylchalcone Production

摘要

2',4' -Dihydroxy-6 -methoxy-3 ',5 '-dimethylchalcone (DMC), a bioactive flavonoid isolated from Cleistocalyx operculatus (Roxb.) Merr and Perry (Myrtaceae) exhibits significant anti-cancer effects and has received great attention recently. In this work, a new endophytic DMC-producing fungus, Ceriporia lacerata DMC 1106, was isolated from the bud of C. operculatus. Its identity was confirmed based on rDNA ITS sequences (ITS1 and ITS2 regions and the intervening 5.8S rDNA region) and phylogenetic analysis. Furthermore, statistical screening designs were applied to identify significant medium variables for DMC production and to find their optimal levels. The difference between the optimized medium and the original medium suggested that L-phenylalanine, magnesium ion and oxalic acid might be attributed to the enhancement of DMC production. Compared with the initial medium, the production of DMC was increased 6-fold in optimum medium. These results demonstrated that the endophytic fungus Ceriporia lacerata DMC1106 has potential applications for DMC production.