Investigation of the potential of agro-industrial material as low cost substrate for ethanol production by using Candida tropicalis and Zymomonas mobilis

摘要

Ethanol production from agro-industrial waste (thippi) was analyzed as low-cost feedstock using mixed culture fermentation. Reducing sugars yield by acid hydrolysis was 395.2 gkg~(-1) thippi and ethanol yield was 35.8gkg~(-1) thippi. However, reducing sugars in enzyme hydrolysis was 535.33 gkg~(-1) thippi, which resulted in 254.45 gethanol yield kg~(-1) thippi (72.8 gl~(-1), using mixed culture), revealed 93.18% of the theoretical yield of ethanol. Zymomonas mobilis alone yielded 228.55 gethanolkg~(-1) thippi (65.3gl~(-1)), which is 82.83% of the theoretical yield and Candida tropicalis alone yielded 214.2 g ethanol kg~(-1) thippi (61.2 g ethanoll~(-1)), reaching more than the published value for C. tropicalis with starch. This is 76.58% of the theoretical yield. These data are of 11 fermentor. Ethanol fermentation from thippi hydrolysate was then scaled up to 101 fermentor; ethanol yield was 219.63 gkg~(-1) thippi, which was 78.82% of the theoretical yield.