Human endo-α1,2-mannosidase is a Golgi-resident type Ⅱ membrane protein

摘要

The cDNA for human endo-α1,2-mannosidase was reconstructed using two independent EST-clones and its properties characterized. The 2837 bp cDNA construct contained a 1389 bp open reading frame (ORF) encoding for 462 amino acids and a ～53.6 kDa protein, respectively. Hydrophobicity analysis of this amino acid sequence, as well as proteolytic degradation studies, indicate that the enzyme is a type Ⅱ protein, anchored in the membrane via a 19 amino-acid long apolar sequence close to the N-terminus. Human endo-α1,2-mannosidase displays a high degree of sequence identity with the catalytic domain of the homologous rat liver endo-enzyme, but differs substantially in the N-terminal peptide region, which includes the transmembrane domain. No sequence similarity exists with other processing α-glycosidases. Based on sequence information provided by the 2837 bp construct, the cDNA consisting of the complete 1389 bp ORF was amplified by RT-PCR using human fibroblast RNA. Incubation of E. coli lysates with this cDNA, previously modified for boost translation by codon optimization, resulted in the synthesis of a ～52 kDa protein which degraded [~(14)C]Glc_3-Man_9-GlcNAc_2 efficiently, indicating that the catalytic domain of the enzyme folds correctly under cell-free conditions. Transfection of the endo-α1,2-mannosidase wild-type cDNA into COS 1 cells resulted in a moderate (～1.5-fold) but reproducible increase of activity compared with control cells, whereas > 18-fold increase in activity was measured after expression of a chimera containing green-fluorescent-protein (GFP) attached to the N-terminus of the endo-α1,2-mannosidase polypep-tide. This, together with the observation that GFP-endo-α1,2-mannosidase is expressed as a Golgi-resident type Ⅱ protein, points to enzyme-specific parameters directing folding and membrane anchoring, as well as Golgi-targeting, not being affected by fusion of GFP to the endo-α1,2-mannosidase N-terminus.