Evaluation of Antibacterial Activity of Macroalgae Extracts as Adjunctive Therapy in Neonates Sepsis Induced by Klebsiella pneumoniae

摘要

Background The greatest challenge of the day is the rising threat of neonatal sepsis as well as antimicrobial resistance. Accordingly, attention has been especially devoted to marine macroalgae metabolites, which are known to have prominent medicinal propertieswith major phytoconstituents. The object of this study is to evaluate the efficacy of three different macroalgal extracts (ME) as new ingredients classes exposed againstmultidrug-resistant (MDR) Klebsiella pneumoniae isolated from blood specimens of neonatal sepsis, as well as to evaluate the bio-compounds present in ME using gas chromatography-mass spectrometry (GC-MS). Materials and Methods Out of 370 neonates, only patients with positive blood culture 110 (29.7%) were enrolled. From clinical specimens, Klebsiella pneumoniae (n = 40) were recovered and were biochemically identified. Antimicrobial susceptibilities of the selected isolates to different antimicrobials were conducted using disk diffusion methods. Antibacterial activities of different AE, Ulva fasciata, Sargassum vulgare, Corallina officinalis, were investigated using agar well diffusion method. Furthermore, the constituents of the most promising ME were detected using GC-MS analysis. Results Susceptibility testing showed that all K. pneumoniae isolates were MDR with a great prevalence of resistance, especially to ampicillin and amoxicillin/clavulanic acid. Methanolic extract of S. vulgare exhibited the highest antibacterial activities. GC-MS data of the selected extract revealed eleven different secondary metabolites, which were mostly composed of organic acids. Conclusion The methanolic extract of the marine alga S. vulgare could be a potential candidate for the natural compounds as antibacterial therapy.