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Radiofrequency encoded angular-resolved light scattering

机译：射频编码角分辨光散射

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摘要

The sensitive, specific, and label-free classification of microscopic cells and organisms is one of the outstanding problems in biology. Today, instruments such as the flow cytometer use a combination of light scatter measurements at two distinct angles to infer the size and internal complexity of cells at rates of more than 10 000/s. However, by examining the entire angular light scattering spectrum, it is possible to classify cells with higher resolution and specificity. Current approaches to performing these angular spectrum measurements all have significant throughput limitations, making them incompatible with other state-of-the-art flow cytometers. Here, we introduce a method for performing complete angular scattering spectrum measurements at high throughput combining techniques from the field of scattering flow-cytometry and radiofrequency communications. Termed Radiofrequency Encoded Angular-resolved Light Scattering, this technique multiplexes angular light scattering in the radiofrequency domain, such that a single photodetector captures the entire scattering spectrum from a particle over approximately 100 discrete incident angles on a single shot basis. As a proof-of-principle experiment, we use this technique to perform scattering measurements over a range of 30° from a tapered optical fiber at a scan rate of 250 kHz.
机译：微观细胞和生物的敏感，特异和无标签分类是生物学中的突出问题之一。如今，诸如流式细胞仪之类的仪器在两个不同的角度结合使用光散射测量，以超过10 000 / s的速率推断细胞的大小和内部复杂性。但是，通过检查整个角度光散射光谱，可以以更高的分辨率和特异性对细胞进行分类。当前执行这些角谱测量的方法都具有明显的吞吐量限制，从而使其与其他最新的流式细胞仪不兼容。在这里，我们介绍了一种结合散射流式细胞术和射频通信领域的技术，以高通量执行完整的角散射光谱测量的方法。这项技术被称为射频编码角分辨光散射，它在射频域中对角光散射进行了多路复用，因此单个光电探测器可以在单次发射的基础上捕获大约100个离散入射角上的粒子的整个散射谱。作为原理验证实验，我们使用此技术以250 kHz的扫描速率在锥形光纤的30°范围内执行散射测量。

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  • 来源
    《Applied Physics Letters》 |2015年第12期|123701.1-123701.4|共4页
  • 作者

    Brandon W. Buckley; Najva Akbari; Eric D. Diebold; Jost Adam; Bahram Jalali;

  • 作者单位

    Department of Physics, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,Department of Electrical Engineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA;

    Department of Electrical Engineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA;

    Department of Electrical Engineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,Department of Bioengineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,California NanoSystems Institute, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA;

    Department of Electrical Engineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,California NanoSystems Institute, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,The Mads Clausen Institute, University of Southern Denmark, Alsion 2, DK-6400 Sonderborg, Denmark;

    Department of Electrical Engineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,Department of Bioengineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,California NanoSystems Institute, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA,Department of Surgery, David Geffen School of Medicine, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
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  • 正文语种 eng
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