Simultaneous Detection of Multiple Biomarkers with over Three Orders of Concentration Difference Using Phase Change Nanoparticles

摘要

A big challenge for multiplexed detection of cancer biomarkers is that biomarker concentrations in body fluid differsnseveral orders of magnitude. Existing techniques are not suitable to detect low- and high-concentration biomarkers (protein andnDNA) at the same time, and liquid chromatography or electrophoresis is used to separate or purify target biomarkers before analysis.nThis paper describes a new broad-range biomarker assay using solid to liquid phase change nanoparticles, where a panel of metallicnnanoparticles (i.e., metals and eutectic alloys) are modified with a panel of ligands to establish a one-to-one correspondence andnattached onto ligand-modified substrates by forming sandwiched complexes. The melting peak and fusion enthalpy of phase changennanoparticles during thermal analysis reflect the type and concentration of biomarkers, respectively. The thermal readout conditionncan be adjusted in such a way that multiple biomarkers with concentration difference over 3 orders of magnitude have beennsimultaneously detected under the same condition.