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Enlightening the malaria parasite life cycle: bioluminescent Plasmodium in fundamental and applied research

机译:启发疟疾寄生虫的生命周期:生物发光疟原虫在基础和应用研究中

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摘要

The unicellular protozoan parasites of the genus Plasmodium impose on human health worldwide the enormous burden of malaria. The possibility to genetically modify several species of malaria parasites represented a major advance in the possibility to elucidate their biology and is now turning laboratory lines of transgenic Plasmodium into precious weapons to fight malaria. Amongst the various genetically modified plasmodia, transgenic parasite lines expressing bioluminescent reporters have been essential to unveil mechanisms of parasite gene expression and to develop in vivo imaging approaches in mouse malaria models. Mainly the human malaria parasite Plasmodium falciparum and the rodent parasite P. berghei have been engineered to express bioluminescent reporters in almost all the developmental stages of the parasite along its complex life cycle between the insect and the vertebrate hosts. Plasmodium lines expressing conventional and improved luciferase reporters are now gaining a central role to develop cell based assays in the much needed search of new antimalarial drugs and to open innovative approaches for both fundamental and applied research in malaria.
机译:疟原虫属的单细胞原生动物寄生虫给全世界的人类健康带来了巨大的疟疾负担。对几种疟疾寄生虫进行基因改造的可能性代表了阐明其生物学可能性的重大进展,目前正将转基因疟原虫的实验室品系转变为对抗疟疾的宝贵武器。在各种经过基因修饰的疟原虫中,表达生物发光报告基因的转基因寄生虫系对于揭示寄生虫基因表达的机制以及开发小鼠疟疾模型的体内成像方法至关重要。主要是人类疟疾寄生虫恶性疟原虫和啮齿类寄生虫伯氏疟原虫经过了工程改造,可以在昆虫和脊椎动物宿主之间复杂的生命周期中,在寄生虫的几乎所有发育阶段中表达生物发光报告基因。表达常规和改良的萤光素酶报告基因的疟原虫系现在在开发基于细胞的检测方法中发挥着中心作用,从而迫切需要寻找新的抗疟药,并为疟疾的基础研究和应用研究提供创新方法。

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