Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus

摘要

Molecular ( – ) and biological ( – ) changes induced by tetraspanin1(EgTSP1)-specific dsRNA in different developmental stages of using electro-soaking (ES) and electroporation (EP) compared to the untreated (Ctrl) and the irrelevant dsRNA (Ctrl-irr) controls. EgTSP1 expression profile in different developmental stages of ; invaginated protoscoleces (PSCi), evaginated protoscoleces (PSCe), one-proglottid worms (SW1) and three or more proglottids worms (SW3). – Suppression of EgTSP1 in protoscoleces (PSC), one-proglottid worms (SW1) and three or more proglottids worms (SW3) by dsRNA on days 3, 7 and 14. Viability changes of protoscoleces treated with EgTSP1-specific dsRNA. Evagination rate of protoscoleces treated with EgTSP1-specific dsRNA. , Changes in body contractions per minute in one-proglottid worms (SW1) and three or more proglottids worms (SW3) treated with EgTSP1-specific dsRNA. For Ctrl-irr EgTSP1 expression data are only demonstrated for electro-soaking method. Bars show the mean ± SE. *  P P P