B cells induced by Schistosoma japonicum infection display diverse regulatory phenotypes and modulate CD4+ T cell response

摘要

Infection with alters splenic and PerC B-cell composition. Splenic and PerC lymphocytes were isolated from mice at zero (uninfected mice, 0W), three (3W), eight (8W), and twelve weeks (12W) post-infection. Gating schemes for PerC B-1a cells ( ) and splenic B-cell subsets ( ) within the CD19 gated B-cell population are shown. Splenic B-cell subpopulations ( ) are shown: B-1a cells (CD19 CD5 ), T1 B cells (CD19 CD5 CD21 CD23 ), T2-MZP cells (CD19 CD5 CD21 CD23 ), MZB cells (CD19 CD5 CD23 CD21 ) and FOB cells (CD19 CD5 CD23 CD21 ). The percentages of PerC B-1a cells ( ) were measured. The percentages ( ) and the absolute numbers ( ) of splenic B cell subsets were measured from at least five mice of each time point after infection. All data are representative of at least two independent experiments. *  P P