Objective. After filtration through glomeruli, β2-microglobulin is reabsorbed in proximal tubules. Increased urinary β2-microglobulin indicates proximal tubule injury and measurement of β2-microglobulin in urine is useful to determine the source of renal injury. Kidney injury molecule-1 (KIM-1) has been characterized as a selective proximal tubule injury marker. This study was designed to evaluate the correlation of urinary β2-microglobulin concentration and KIM-1 expression as evidence of proximal tubule injury. Methods. Between 2009 and 2012, 46 patients with urine β2-microglobulin (RenalVysion) had follow-up kidney biopsy. Diagnoses included glomerular and tubule-interstitial disease. Immunohistochemical staining for KIM-1 was performed and the intensity was graded from 0 to 3+. Linear regression analysis was applied to correlate the values of urinary β2-microglobulin and KIM-1 staining scores. P < 0.05 was considered statistically significant. Results. Thirty patients had elevated urinary β2-microglobulin. KIM-1 staining was positive in 35 kidney biopsies. There was a significant correlation between urinary β2-microglobulin and KIM-1 staining (P < 0.05). Sensitivity was 86.6%, specificity was 43.7%, positive predictive value was 74.2%, and negative predictive value was 63.6%. Conclusion. Increased urinary β2-microglobulin is significantly correlated with KIM-1 staining in injured proximal tubules. Measurement of urine β2-microglobulin is a sensitive assay for proximal tubule injury.
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机译：目的。通过肾小球过滤后，β2-微球蛋白在近端小管中重新吸收。尿中β2-微球蛋白升高表明近端小管损伤，尿液中β2-微球蛋白的测定可用于确定肾损伤的来源。肾损伤分子1（KIM-1）已被表征为选择性近端肾小管损伤标记物。本研究旨在评估尿中β2-微球蛋白浓度与KIM-1表达的相关性，作为近端肾小管损伤的证据。方法。在2009年至2012年之间，对46例尿β2-微球蛋白（RenalVysion）患者进行了肾脏活检。诊断包括肾小球和肾小管间质疾病。对KIM-1进行了免疫组织化学染色，强度从0到3+分级。应用线性回归分析来关联尿β2-微球蛋白值和KIM-1染色评分。 P <0.05被认为具有统计学意义。结果。 30例患者尿β2-微球蛋白升高。 35例肾脏活检中KIM-1染色呈阳性。尿β2-微球蛋白与KIM-1染色之间存在显着相关性（P <0.05）。敏感性为86.6％，特异性为43.7％，阳性预测值为74.2％，阴性预测值为63.6％。结论。尿β2-微球蛋白升高与受伤的近端小管中的KIM-1染色显着相关。尿液中β2-微球蛋白的测定是对近端小管损伤的敏感测定。
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