Synchronous Cultures of Bacillus subtilis Obtained by Filtration with Glass Fiber Filters

摘要

A simple method of potentially wide applicability for obtaining synchronous cultures of Bacillus subtilis based on size selection is described. Using glass fiber filters, a population (about 1 to 2% of the parent population) can be obtained substantially enriched for small cells which grow synchronously. A method for rapidly concentrating dilute suspensions of cells is described.