Suspension of Amorphous Calcium Phosphate Nanoparticles Impact Commitment of Human Adipose-Derived Stem Cells In Vitro

摘要

Calcium phosphate is an important component in natural bone. Bone defects caused by trauma or resection of tumors demand bone substitutes to close the defect. One viable option is to use a scaffold material seeded with stem cells. Stem cells can differentiate towards bone cells (osteoblasts), among other cell types. To trigger stem cells towards the osteoblast type of cell, particular supplementation of the culture medium is needed. This study tests whether calcium phosphate nanoparticles can induce a commitment towards the osteoblast type of cell. In addition, we test for other commitments, such as endothelial cell, chondrocyte, or adipocyte commitment. After 1 or 2 weeks, with either 5 or 50 µg/mL nanoparticles in the culture medium, gene expression is analyzed. We find a significant increase of two specific bone marker genes after two weeks in 50 µg/mL nanoparticles compared to 5 µg/mL for two out of three tested human donors of adipose-derived stem cells. Moreover, endothelial cell commitment is also induced. Hence, such nanoparticles have the potential to trigger osteogenic and endothelial cell commitment.