Meta-Transcriptome Profiling of Novel Invasive Pest Spodoptera frugiperda in Yunnan China

摘要

A Distribution of assembled transcripts with different length. B Distribution of sequencing depth. Bar graph representation of sequencing depth versus the percentage of transcripts having the respective depth. C A summary of transcripts annotation. The left pie shows the proportional distribution of transcripts with/without homologous reference in nr/trEMBL database. The right pie shows a detail distribution for taxon of bacteria, plant, insect, fungus, viruses and unassigned. D The top 8 taxons involving bacterias from matagenomic sequencing were presented. E Taxons involving plants from matagenomic sequencing were presented. F PCR confirmation of the detected viruses from RNA-Seq analysis in the sample. G Phylogenetic analysis of S. frugiperda. Maximum-Likelihood phylogenetic tree of S. frugiperda was constructed based on the COX I gene using Busseola spp. as outgroups. Sequences derived from RNA-seq were highlighted with a red solid circle. H Comparison of the portion of the S. frugiperda Tpi gene with Corn and Rice Strain. Strain-specific polymorphic sites were highlighted with red triangle.