Unexpected effects of ivermectin and selamectin on inducible CreER activity in mice

摘要

Ivermectin-induced silencing of Ela1-CreER in the pancreas. Representative immunofluorescent images of postnatal day 21 pancreata. Sections are labeled with antibodies against amylase (red) for acinar cells and YFP (green) for expression of the Rosa26lsl-YFP reporter of Cre activity. Colabeled with DAPI (blue, faint) for nuclei. Genotype and treatment group are indicated above images. No groups were administered tamoxifen at any time. Column 1 shows no YFP expression in untreated mice lacking the Ela1-CreER transgene (3/3 mice analyzed). Column 2 shows constitutive Ela1-CreER activity in untreated mice (7/7 double transgenic mice analyzed). Column 3 shows constitutive Ela1-CreER activity in spite of ivermectin treatment (2/6 double trangenic mice analyzed) whereas column 4 shows lost constitutive Ela1-CreER activity following ivermectin treatment (4/6 double transgenic mice analyzed)