Tamoxifen-inducible cardiac-specific Cre transgenic mouse using VIPR2 intron

摘要

Generation of VIPR2-ERT2CreERT2 transgenic mice and analysis of transgene expression. a Schematic structure of VIPR2-ERT2CreERT2 transgene. Numbers indicate exons of human VIPR2 gene. Arrowheads indicate primers for genotyping. b PCR genotyping of representative founder mice showing a 276-bp PCR product specific for transgene and a 159-bp product for endogenous gene (+/+, wild-type; +/Tg, heterozygous transgenic mouse). c Strategies for treating pregnant mice with tamoxifen followed by LacZ staining. d Whole-mount LacZ staining of VIPR2:R26 compound heterozygotes E11.5 embryos. Each number indicates VIPR2-ERT2CreERT2 transgenic mouse line. Arrows indicate heart-specific X-gal staining. Original magnification, 20 ×