Characterization of changes in global gene expression in the hearts and kidneys of transgenic mice overexpressing human angiotensin-converting enzyme 2

摘要

Reverse tet promoter-controlled transactivator (rtTA) and hACE2 vectors and identification of α-MHC/rtTA and TRE/hACE2 transgenes. a Construction of the pα-MHC/rtTA and pTRE/hACE2 expression vectors. rtTA and hACE2 (human ACE2) were placed under the control of the α-MHC and TRE promoter, respectively. Two independent lineages of transgenic (Tg) mice were produced and mated together to obtain the double Tg (dTg) mice. In the dTg mice, the expression of the hACE2 is induced by rtTA in the presence of doxycycline (Dox). The arrow (--->) indicates transcription. b Features of the tet response element (TRE) promoter sequence. The TRE promoter is contained with seven copies of the tetO sequence, a TATA box, and an hCMV promoter. c The genomic DNA was isolated from the tail of the founder mouse, and the 174-bp and 545-bp products were shown in the dual transgenic mice carrying the α-MHC/rtTA and TRE/hACE2 transgenes, respectively