Evolving applications of the egg: chorioallantoic membrane assay andex vivo organotypic culture of materials for bone tissueengineering

摘要

The chick chorioallantoic membrane model has been around for over a century,applied in angiogenic, oncology, dental and xenograft research. Despite itsoften perceived archaic, redolent history, the chorioallantoic membrane assayoffers new and exciting opportunities for material and growth factor evaluationin bone tissue engineering. Currently, superior/improved experimentalmethodology for the chorioallantoic membrane assay are difficult to identify,given an absence of scientific consensus in defining experimental approaches,including timing of inoculation with materials and the analysis of results. Inaddition, critically, regulatory and welfare issues impact upon experimentaldesigns. Given such disparate points, this review details recent research usingthe ex vivo chorioallantoic membrane assay and the exvivo organotypic culture to advance the field of bone tissueengineering, and highlights potential areas of improvement for their applicationbased on recent developments within our group and the tissue engineeringfield.