Coumarins content in wine: application of HPLC fluorescence spectrometry and chemometric approach

摘要

HPLC chromatograms standard mixture of coumarins (a) and Slovak Tokaj wines (b sample 2, c sample 4, d sample 7). Chromatographic conditions: Kinetex C18 (100 mm × 4.6 mm I.D., 5 μm particle size) column, gradient elution with methanol-1% acetic acid as mobile phase, flow rate 1.0 mL min−1, column temperature 23 °C, Fluorescence detection at 330 nm (λex) and 450 nm (λem)