Cell number considerations for blastocyst transfer in younger patients

摘要

Photographic example for each ICM grade. The ICM grades of a, b, c, d, e, f, and g were 7, 6, 5, 4, 3, 2, and 1, respectively. The ICM diameters of a, b, c, d, e, and f were 82.3, 83.3, 68.3, 61.8, 41.5, and 41.8 μm, respectively. The ICM diameter was defined as the average value of the longest and shortest diameter. In all panels, bar  =  20 μm. The details of ICM scoring criteria are as follows: 7, ICM diameter is > 70 μm, cells compacted, tightly adhered together and indistinguishable as individual cells; 6, ICM diameter is > 70 μm, some cells less compacted, loosely adhered together, some individual cells are visible; 5, ICM diameter is 50–70 μm, cells compacted, tightly adhered together and indistinguishable as individual cells; 4, ICM diameter is 50–70 μm, some cells less compacted, loosely adhered together, some individual cells are visible; 3, ICM diameter is 30–50 μm, cells compacted, tightly adhered together and indistinguishable as individual cells; 2, ICM diameter is 30–50 μm, some cells less compacted, loosely adhered together, some individual cells are visible; 1, very few cells, either compacted or loose, may be difficult to completely distinguish from trophectoderm cells or fragmentations