Single-cell RNA-seq analysis reveals ploidy-dependent and cell-specific transcriptome changes in Arabidopsis female gametophytes

摘要

Schematic diagram of scRNA-seq analysis in diploid and tetraploid plants. a Relative expression level per transcriptome and absolute transcript number per cell for a gene (red) in a diploid and a tetraploid cell. b Pipeline of scRNA-seq analysis. Each color indicates one type of unique molecular identifiers (UMIs). ERCC, External RNA Controls Consortium. The absolute transcript level per gene is determined by the number of distinct UMIs aligned to each transcript, excluding PCR duplets, which is 2 for gene X, 3 for gene Y, and 2 for one ERCC RNA