Precise annotation of tick mitochondrial genomes reveals multiple copy number variation of short tandem repeats and one transposon-like element

摘要

Long-PCR amplification of each entire mt genome. All the primers and PCR reaction conditions are listed in Table ​Table1.1. The tRNA genes are represented by their single letter codes. CR1 and CR2 represents the control region 1 and the control region 2, respectively. Translocated genes are reported in the same colour. All the reference genomes of tick mitochondria read in the 5′ → 3′ direction as the major coding strand (J-strand). Genes on the J-strand and the N-strand are shown high and low, respectively. a. The tick mt genomes were classified into three types, which are type I, II and III (Results). The type III mt genomes were amplified into two large segments (L1&L2 or L3&L4) by long-PCR using total DNA from individual ticks. Using the complete D. silvarum mt genome (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"MN347015","term_id":"1800329081","term_text":"MN347015"}}MN347015), L1, L2, L3 and L4 were estimated as ~ 9.6, ~ 8.2, ~ 7.2 and ~ 9.2 Kbp in size (Table ​(Table1),1), respectively. b. The type III mt genomes of ticks read clockwise in the 5′ → 3′ direction