High fat diet induced obesity model using four strains of mice: KunmingC57BL/6 BALB/c and ICR

摘要

High fat diet (HFD) treated mouse is widely used as experimental animal model forhyperlipidemia and hyperglycemia study. Many factors contribute to establish animal modelthat meant to simulate high fat and glucose diet induced phenotypes. In the present study,four strains of experiment mouse treated by HFD were used to explore the impact of mousestrain on lipid profile, glucose level, and major inflammation cytokines. HFD fed Kunmingand ICR mouse gained significantly higher body weight than control which was not shown byC57BL/6 and BALB/c mouse. All four strains fed by HFD has heavier liver and adipose tissuethan control ones. Obvious fat droplets and enlarged adipose cells were observed in obesemouse of four strains. Additionally, obese mouse showed typical response to glucose andinsulin load in OGTT and ITT. Serum TC, LDL-c, and TC/HDL-c ratio, but not TG, increasedin all four strains. Major inflammatory cytokines and insulin level showed little changesin obese mouse as well ( <0.05) The present study could provide basicinformation for diet induced obesity developed by four commonly used experimental mousestrains.