Methodology and application of PCR‐RFLP for species identification in tuna sashimi

摘要

The Thunnini, or tuna, comprise many species with very different commercial values. The principal raw tuna product on the market is sashimi, for which the species used is difficult to identify through conventional morphological analysis. The present study amplified the cytochrome gene ( ) of 4 major tuna species used for preparing sashimi—yellowfin tuna ( ), southern bluefin tuna ( ), bigeye tuna ( ), and Atlantic bluefin tuna ( )—and 4 species commonly mislabeled as components of tuna sashimi—albacore tuna ( ), skipjack tuna ( ), striped marlin ( ), and swordfish ( ). Polymerase chain reaction (PCR) amplicons were digested with 5 restriction enzymes— 147 I, I, I, I, and II—to obtain characteristic restriction maps of the above‐mentioned raw tuna species and the commonly mislabeled species. An identification method using PCR restriction fragment length polymorphism (PCR‐RFLP) was established and validated using 39 commercial tuna sashimi samples, which verified that this method provides results consistent with those obtained by classical sequencing. PCR‐RFLP has several advantages over classical sequencing, such as simplicity, speed and accuracy. This technique could support species identification for raw tuna and sashimi.