The plant terpenoid carvone is a chemotaxis repellent for C. elegans

摘要

Chemotaxis assays were performed on 35 mm circular plates divided into quadrants. Behavior in the N2 wild type strain was scored in response to experimental compounds (E, plant compounds dissolved in ethanol) and solvent (vehicle control, 100% ethanol). Chemotaxis behavior was neutral for solvent and most plant compounds, including eugenol (pink), cis-jasmone (light orange), linalool (dark orange), and (–)-ɑ-thujone (green). Worms were repulsed by S-(+)-carvone (dark blue). Groups not connected by the same letter are significantly different. Chemotaxis behavior was scored across divergent worm strains in response to S-(+)-carvone (dark blue), solvent (yellow) and (–)-ɑ-thujone (green). There was a significant effect of compound, but not strain, on chemotaxis response. Chemotaxis behavior in response to stereoisomers of carvone, including S-(+)-carvone (dark blue) and R-(–)-carvone (light blue), was measured in wild type (N2) and two chemosensation mutant strains, and . Groups not connected by the same letter are significantly different.