TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes

摘要

The TRF3 expression level is enhanced during ME differentiation of hESCs. Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts.  = 3 each. qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM.  = 3 each. *  p