High-throughput method for detection and quantification of lesions on leaf scale based on trypan blue staining and digital image analysis

摘要

Overview of the lesion detection and quantification method. There are two main steps (1) damage detection and visualisation by clearing and staining and subsequent microscopy verification of staining (scale bar 50 µm) and image acquisition; and (2) damage quantification using the LiMu image analysis program. LiMu program results were (3) evaluated by comparing them against results acquired with IMAGEJ software and manual assessment, and validated with IMAGEJ results, followed by (4) application of the method on an experimental dataset