Development of the sequence-characterized amplified region (SCAR) marker for distinction of intergeneric hybrids between Argyranthemum frutescens (L.) Sch. Bip. and Rhodanthemum gayanum (Cross. Durieu) B.H. Wilcox K. Bremer Humphries

摘要

Distinction of intergeneric hybrids between (L.) Sch. Bip. and (Cross. & Durieu) B.H. Wilcox, K. Bremer & Humphries is important for the development of new intergeneric hybrids. The aim of this study was to develop a simple and cost-effective DNA marker to distinguish between a large number of putative hybrids of × . during primary selection. We designed sequence-characterized amplified region (SCAR) markers based on the genetic sequences of the internal transcribed spacer (ITS) region for and . . Specific SCAR markers for amplified bands in and hybrids but not in . In contrast, specific SCAR markers for amplified bands in and hybrids but not in . Additionally, we confirmed the reproducibility of the SCAR marker using 31 cultivars. The findings demonstrated that these SCAR markers could be used to distinguish putative hybrids between and . . This is the first report of the development of a SCAR marker to facilitate distinction of these hybrids.