Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

摘要

Schematics of in vivo AO-TPEFM of the mouse retina using direct wavefront sensing from a two-photon fluorescence guide star. Representative spot diagram collected by the SHWS. Representative aberration wavefront of the mouse eye (unit: μm). , The lateral ( ) and axial ( ) resolution of the AO-TPEFM system after full AO correction. The mean values of the measured FWHM from six locations are shown in the figures. Depth-resolved imaging of different retinal layers with system (top) or full (bottom) AO correction. Mosaic TPEF images of RGCs (green) and blood vessels (red) with system (left) and full (right) AO correction. Signal profiles along the dashed lines in ( ) for a comparison of the fluorescence intensity of RGCs with system (red line) and full (black line) AO correction