Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome

摘要

Schematic diagram of the SR-FACT setup. Flow chart of the ODT reconstruction algorithm. Flow chart of the SIM reconstruction algorithm. Representative example of a COS-7 cell labeled with LifeAct-EGFP imaged with SR-FACT. Scale bar, 5 μm. Average intensity profiles of RIs (orange) and fluorescence (green) along lines orthogonal to the actin filaments (with the same filament at 50 time points); a representative example is shown in ( ). Average full width at half maximum (FWHM) values of the profiles orthogonal to the actin filament labeled by the lines in ( ) measured at 50 different time points. Center lines, medians; limits, 75 and 25%; whiskers, maximum and minimum.