Heat shock protein 70 increases cell proliferation neuroblast differentiation and the phosphorylation of CREB in the hippocampus

摘要

Purification and transduction of the Tat-HSP70 protein into the mouse hippocampus. Overview of the Tat-HSP70 protein. Expression and purification of the Tat-HSP70 and control-HSP70 proteins, as assessed by western blot analyses using an anti-polyhistidine antibody. Western blot analysis of polyhistidine in the hippocampal homogenates of control, vehicle (10% glycerol)-, control-HSP70-, and Tat-HSP70-treated groups (  = 5 per group;  b  c  d Immunohistochemistry for HSP70 in the dentate gyrus of control, vehicle-, control-HSP70-, and Tat-HSP70-treated mice. Note that HSP70 immunoreactive structures are most abundantly found in the Tat-HSP70-treated group. GCL, granule cell layer; ML, molecular layer; PL, polymorphic layer. Scale bar = 50 μm