Decrease of 14–3-3 proteins by glutamate exposure in the cerebral cortex of newborn rats

摘要

Hematoxylin and eosin stained photomicrographs ( - ), 14–3-3 β/α, 14–3-3 ζ/δ, 14–3-3 γ, and 14–3-3 ε protein spots ( and ), and graph of each spots intensity ( - ) from neonatal cerebral cortices, of vehicle- ( , and ) and glutamate treated ( , and ) neonatal rats. Among 6 layers of cerebral cortex, external pyramidal layer (III) was observed ( and ). Arrows indicate normal neurons with well-developed dendrites and typical pyramidal shape ( ). Arrowheads indicate damaged neurons with shrunken dendrites and atypical round shape ( ). Scale bar: 50 μm. Arrows and numbers indicate 14–3-3 proteins spots ( and ). (1) 14–3-3 β/α, (2, 3) 14–3-3 ζ/δ, (4) 14–3-3 γ, (5) 14–3-3 ε. Spot intensities were measured using PDQuest software. The spot intensities are reported as a ratio relative to control animals. Data (  = 4) are shown as mean ± S.E.M. *