Effect of Different Concentrations of Forskolin Along with Mature Granulosa Cell Co-Culturing on Mouse Embryonic Stem Cell Differentiation into Germ-Like Cells

摘要

Germ cell development processes are influenced by soluble factors and intercellular signaling events between them and the neighboring somatic cells. More insight into the molecular biology of the germ cell development from ES cells and investigation of appropriate factors, specifically those targeting differentiation processes, is of great importance. In this study, we established an model with higher ES cell differentiation rate to germ cells, using adenylate cyclase activator, forskolin.