A detached petal disc assay and virus-induced gene silencing facilitate the study of Botrytis cinerea resistance in rose flowers

摘要

Petal discs were inoculated with different concentrations of conidia. Disease lesions were observed at 12, 24, 36, 48, 60, and 72 hpi. Statistical analysis of disease lesions following inoculation with different concentrations of conidia. The graph shows the average lesion size from three biological replicates (  = 48) with the standard deviation. Significant differences are indicated by lowercase letters according to Duncan’s multiple range test (  c Quantification of cell death triggered by infection of rose petal discs. The graph shows ion leakage values measured at 12, 24, 36, 48, 60, and 72 hpi. The ion leakage changes resulting from petal cell death were consistent with the disease lesion diameter. This experiment was repeated two times with similar results.