The micropeptide LEMP plays an evolutionarily conserved role in myogenesis

摘要

Deep-sequencing signals of MyolncR4. Numbers to the left show the RPM. SC satellite cell, MT myotube, cyto cytoplasm, nuc nucleus. The regions are boxed in color is the most conserved part of MyolncR4. RT-qPCRs to examine the expression level of MyolncR4 in satellite cells and differentiated myotubes. The bars show RNA levels relative to GAPDH. Error bars, standard deviations (  = 3). Statistical analysis was performed using Student’s test. ***  c RT-qPCRs to examine the expression level of MyolncR4 during C2C12 differentiation. The bars show RNA levels relative to GAPDH. Error bars, standard deviations (  = 3). Statistical analysis was performed using Student’s test. *  d (Top): Diagram of constructs used for transfection. (Bottom): Western blot to examine LEMP expression in HeLa cells transfected with LEMP-HA or Flag-LEMP. Illustration of Flag knock-in strategy. , Western blot and IF to detect the expression of Flag-LEMP in Flag KI cells using the Flag antibody. Western blot to examine the expression of endogenous LEMP with an antibody raised against the C-terminal region of LEMP. The asterisks indicate nonspecific bands that are detected by the LEMP antibody.