DPF is a cell-density sensing factor with cell-autonomous and non-autonomous functions during Dictyostelium growth and development

摘要

Conditioned media promotes aggregation at low cell density. Log phase growing WT cells were adhered in a 12-well plate under DB starvation buffer at indicated cell densities for 24 h, using either fresh, naïve DB media, or cell-free, > 30-kDa conditioned media from WT cells starved in DB for 18 h (see also Additional file : Figure S1A,B)