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Correlation Analysis between AK1 mRNA Expression and Inosine Monophosphate Deposition in Jingyuan Chickens

机译:靖远鸡AK1 mRNA表达与肌苷一磷酸沉积的相关性分析

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摘要

China is rich in livestock and poultry resources, but the development of animal husbandry in different regions is uneven, and excellent livestock and poultry resources have to be developed. Jingyuan chicken is a national-level protected breed of livestock and poultry and a genetically protected breed in the Ningxia Hui Autonomous Region. It is rich in amino acids and fatty acids, with a high nutritional value. It is the first choice of “green nutrition and health care” in chicken food. Meat flavor is the main factor affecting meat quality. Meat flavor is characterized by umami and aroma properties. Inosine acid (inosincacid, inosinemonphosphate, IMP) has the greatest influence on meat flavor and is an important indicator of the freshness of meat. The purpose of this study was to screen out differentially expressed genes related to IMP content in different parts of the Ningxia local breed of Jingyuan chicken on the basis of transcriptome sequencing, and select adenylate kinase 1 ( ) for quantitative fluorescence verification and Pearson correlation analysis. These findings provide a theoretical basis for further discovery and screening of IMP-specific deposition regulation mechanisms, as well as scientific basis for the development and utilization of local varieties and the development of new approaches to molecular breeding.
机译:中国拥有丰富的畜禽资源,但不同地区的畜牧业发展不平衡,必须开发优质的畜禽资源。靖远鸡是宁夏回族自治区的国家级畜禽保护品种和转基因品种。它富含氨基酸和脂肪酸,具有很高的营养价值。它是鸡肉中“绿色营养与保健”的首选。肉的味道是影响肉质的主要因素。肉味的特征在于鲜味和香气。肌苷酸(肌苷酸,肌苷磷酸,IMP)对肉类风味的影响最大,并且是肉类新鲜度的重要指标。这项研究的目的是在转录组测序的基础上,筛选靖远鸡宁夏本地品种不同部位与IMP含量相关的差异表达基因,并选择腺苷酸激酶1()进行定量荧光验证和Pearson相关分析。这些发现为进一步发现和筛选IMP特异性沉积调节机制提供了理论基础,也为开发和利用本地品种以及开发新的分子育种方法提供了科学依据。

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