Selenomethionine p-Cyanophenylalanine Pairs Provide a Convenient Sensitive Non-Perturbing Fluorescent Probe of Local Helical Structure

摘要

The use of selenomethionine (MSe)-p-cyanophenylalanine (FCN) pairs to probe protein structure is demonstrated. MSe quenches FCN fluorescence via electron transfer. Both residues can be incorporated recombinantly or by peptide synthesis. Time-resolved and steady-state fluorescence measurements demonstrate that MSe-FCN pairs provide specific local probes of helical structure.