A validated dispersive liquid-liquid microextraction method for extraction of ochratoxin A from raisin samples

摘要

A method based on dispersive liquid-liquid microextraction (DLLME) was developed for the quantitative extraction of Ochratoxin A (OTA) from raisin samples. The influence of various parameters on the recovery of OTA such as type and volume of DLLME extractant, centrifuging and sonication time, also volume of deionized water was investigated. Recovery values under the optimum conditions were between 68.6 and 85.2 %, the inner and intra-day precision expressed as relative standard deviation (RSD%, n = 3), were less than 15 % at spiking levels of 2.5–30 μg kg⁻¹. Linearity was studied from 0.5 to 30 μg L⁻¹, and the limits of detection (LOD) and quantification (LOQ) were 0.7 and 2.0 μg kg⁻¹, respectively. Real samples were analyzed by DLLME method and compared with confirmative immunoaffinity Column Chromatography (IAC) clean-up. Low cost, simplicity of operation, speed and minimum consumption of organic solvent were the main advantages of proposed method. The mean contamination of samples was 0.88 μg kg⁻¹ that was lower than European Legal Limit.