Response of soil microbial community to application of biochar in cotton soils with different continuous cropping years

摘要

The bacterial community in soils of cotton that have continuously been cropped for 2 years, 6 years, 11 years and 14 years and treated with biochar (B0, 0 t·ha⁻¹; B1, 12.5 t·ha⁻¹; and B2, 20 t·ha⁻¹) was investigated using next-generation sequencing. Of the 45 bacterial genera (relative abundance ratio of genera greater than 0.3%), 21 genera were affected (p < 0.05) by the biochar treatment, whereas 20 genera were affected by the continuous cropping. Between the soils that have been continuously cropped for 2 years and 14 years, 12 different genera were significantly observed (p < 0.05), and 6 genera belonged to the phylum Acidobacteria. The relative abundance of Sphingomonas and Pseudomonas in the biochar-treated soils was significantly higher than that in the soil without biochar treatment (p < 0.05), and the relative abundance of Sphingomonas and Pseudomonas in soils that have been continuously cropped for 2 years and 6 years was significantly higher than that in the soils continuously cropped for 11 years and 14 years (p < 0.05). The results suggest that the biochar application has a significant impact on the soil bacterial community, which may improve the microbial diversity of continuous cropping systems in cotton soils.