This report describes the fabrication of a novel microfluidics nanobiochip based on a composite comprising of nickel oxide nanoparticles (nNiO) and multiwalled carbon nanotubes (MWCNTs), as well as the chip's use in a biomedical application. This nanocomposite was integrated with polydimethylsiloxane (PDMS) microchannels, which were constructed using the photolithographic technique. A structural and morphological characterization of the fabricated microfluidics chip, which was functionalized with a bienzyme containing cholesterol oxidase (ChOx) and cholesterol esterase (ChEt), was accomplished using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy. The XPS studies revealed that 9.3% of the carboxyl (COOH) groups present in the nNiO-MWCNT composite are used to form amide bonds with the NH2 groups of the bienzyme. The response studies on this nanobiochip reveal good reproducibility and selectivity, and a high sensitivity of 2.2 mA/mM/cm2. This integrated microfluidics biochip provides a promising low-cost platform for the rapid detection of biomolecules using minute samples.
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机译：这份报告描述了一种新型的微流控纳米生物芯片的制造，以及基于氧化镍纳米粒子（nNiO）和多壁碳纳米管（MWCNT）的复合材料，以及该芯片在生物医学应用中的用途。该纳米复合材料与使用光刻技术构建的聚二甲基硅氧烷（PDMS）微通道集成在一起。使用X射线光电子能谱（XPS）和扫描电子显微镜对所制造的微流控芯片进行结构和形态表征，该芯片已用含有胆固醇氧化酶（ChOx）和胆固醇酯酶（ChEt）的双酶功能化。 XPS研究表明，nNiO-MWCNT复合材料中存在的9.3％羧基（COOH）用于与双酶的NH2基团形成酰胺键。对这种纳米生物芯片的响应研究显示出良好的重现性和选择性，并具有2.2 mA / mM / cm 2 的高灵敏度。这种集成的微流控生物芯片为使用微小样品快速检测生物分子提供了一个有前途的低成本平台。
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