Heat-stable Chemically Defined Medium for Growth of Animal Cells in Suspension

摘要

Procedures are described for preparation of a chemically defined medium, capable of being autoclaved, for the cultivation of animal cells in suspension. Yields of 34.1 × 10⁵, 33.8 × 10⁵, and 47.1 × 10⁵ cells per ml were recorded for cat kidney, HeLa, and mouse fibroblast (L) cells after 6, 10, and 10 days of incubation, respectively.