A transparent plating medium capable of initiating early and rapid growth of the SCHU S4 strain of Pasteurella tularensis is described. On this medium, colonies 1 mm in diameter were obtained following 29 hr of incubation at high humidity. To obtain this growth enhancement, blood factors were provided in the form of a peptic digest of hemoglobin and a saline wash of red blood cells (rabbit). In the basal medium, the presence of both 0.5% l-cysteine-HCl, and sodium thioglycolate, was required to achieve this growth response.
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