The half-width scaling of experimental and model electric field autocorrelation functions of light scattered from normally swimming, defectively swimming, and immotile bull spermatozoa is examined. It is found that a scatter of size 9.0 x 2.3 x 0.45 micrometers is most appropriate for this Rayleigh-Gans-Debye ellipsoid model. In the case of the immotile cells, this model correctly predicts the features seen in the scaling data as well as the absolute value of the data. For the normally swimming and defective populations the model proves to predict correctly the features seen in experimental scaling curves, but not the absolute value of the data. This discrepancy appears to be related to a lack of detail in the model, since the agreement is poorest at large scattering angles.
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机译:检查了从正常游泳,有缺陷的游泳和不运动的公牛精子散射的光的实验和模型电场自相关函数的半角缩放。已经发现,对于这种瑞利-甘斯-德拜椭圆形模型,最合适的散布是9.0 x 2.3 x 0.45微米。对于不能移动的单元格,此模型可以正确预测缩放数据中的特征以及数据的绝对值。对于正常游泳和有缺陷的种群,该模型证明可以正确预测在实验比例曲线中看到的特征,而不是数据的绝对值。这种差异似乎与模型中缺乏细节有关,因为在大散射角处一致性最差。
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