Construction of a restriction-less marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum

摘要

BackgroundClostridium acetobutylicum is a gram-positive, spore-forming, anaerobic bacterium capable of converting various sugars and polysaccharides into solvents (acetone, butanol, and ethanol). The sequencing of its genome has prompted new approaches to genetic analysis, functional genomics, and metabolic engineering to develop industrial strains for the production of biofuels and bulk chemicals.